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Tests and Services Manual: General Bacteriology

ANTIMICROBIAL
SUSCEPTIBILITY TEST-
CONFIRMATION

Specimen:

Isolate

  Method:

E-Test Gradient MIC

Turn Around Time:

24 – 72 hours

Special Requirements:
  • Send pure culture on appropriate media at room temperature
  • ID of organism
  • Antibiotics requested for testing
ANTIMICROBIAL
SUSCEPTIBILITY
TEST – VISA/VRSA
(Vancomycin Intermediate/
Resistant S. aureus)
CONFIRMATION

Specimen:

Isolate

  Method:

E-Test Gradient MIC

Turn Around Time:

24 – 72 hours

Special Requirements:

Send pure culture on appropriate media at room temperature
BACTERIAL CULTURE
(NYC OCME ONLY)

Specimen:

Primary specimens

  Method:
  • Conventional microbiological methods
  • MALDI-TOF

Turn Around Time:

72 hours – 2 weeks

Special Requirements:

Send specimens in sterile container at room temperature
BACTERIAL ISOLATE
FOR IDENTIFICATION

Specimen:

Isolate

  Method:
  • Conventional microbiological methods
  • MALDI-TOF

Turn Around Time:

48 hours – 2 weeks

Special Requirements:

Send pure culture on appropriate media at room temperature
BORDETELLA
PERTUSSIS ID/
CONFIRMATION

Specimen:

Isolates/primary specimens

  Method:
  • Conventional microbiological methods
  • DFA

Turn Around Time:

24 hours to 5 days

Special Requirements:
  • Contact Bureau of immunization prior to submission at (347) 396-2402
  • Send pure culture for isolates on Regan-Lowe agar slant at room temperature
  • Send primary specimens in sterile container.
CAMPYLOBACTER ID/SPP

Specimen:

Isolates

  Method:
  • Conventional microbiological methods
  • MALDI-TOF

Turn Around Time:

48 – 96 hours

Special Requirements:

Send pure culture on Campy BA media at room temperature
SEROTYPING FOR

  1. H. INFLUENZA
  2. L. MONOCYTOGENES
  3. N. MENINGITIDIS

Specimen:

Isolates

  Method:
  • Conventional microbiological methods
  • MALDI-TOF
  • Molecular methods and AST (for N. meningitidis only)

Turn Around Time:

24 – 72 hours

Special Requirements:
  • Bureau of Communicable Disease request only
  • Send pure culture on appropriate media at room temperature
LEGIONELLA
CULTURE - CLINICAL

Specimen:

Isolates/Primary specimens

  Method:
  • Conventional microbiological methods
  • MALDI-TOF
  • DFA

Turn Around Time:

24 hours – 7 days

Special Requirements:
  • Bureau of Communicable Disease request only
  • Send pure culture on appropriate media for isolates at room temperature
  • Send primary specimens in sterile container at room temperature
GC CULTURE
(DOHMH Sexual Health
Clinics Only)

Specimen:

On Jembec media only

  Method:
  • Conventional microbiological methods
  • MALDI-TOF
  • AST for positive culture

Turn Around Time:

24 – 72 hours

Special Requirements:
  • Send on appropriate media at room temperature