Department of Forensic Biology

Forensic Biology Protocols for Mitochondrial DNA Analysis Manuals

This manual contains individual procedures pertaining to Mitochondrial DNA testing of biological specimens.

The procedures on this page apply to versions in effect from January 1, 2025, to the present. The effective dates are indicated in the file name in the format MM/DD/YY - MM/DD/YY. Documents will open in .PDF format.

Note: The most current manual in each section is the first link listed.

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Click or press the enter key on any topic below to access the relevant PDF file of the associated procedure.

Pre-Amplification Procedures

Hair Evidence Examination

Hair Evidence Examination effective 06/20/16 – current

Washing Hair for Mitochondrial or Nuclear DNA Testing

Washing Hair for Mitochondrial or Nuclear DNA Testing effective 09/12/17 – current

Organic Extraction for Mitochondrial or Nuclear DNA Testing

Organic Extraction for Mitochondrial or Nuclear DNA Testing effective 08/23/23 – current

mitoMPS Massively Parallel Sequencing Procedures

Mitochondrial DNA Guidelines for mitoMPS casework

Mitochondrial DNA Guidelines for MitoMPS casework effective 09/06/23 – current

Library Amplification with the PowerSeq CRM kit

Library Amplification with the PowerSeq CRM kit effective 12/21/23 – current

Ampure XP bead-based library purification

Ampure XP bead-based library purification effective 09/19/23 – current

Library Quantification with the PowerSeq Quant MS kit

Library Quantification with the PowerSeq Quant MS kit effective 09/08/23 – current

Normalization of MPS Libraries

Normalization of MPS Libraries effective 09/08/23 – current

mtDNA Sequencing using the MiSeq/MiSeq FGx

mtDNA Sequencing using the MiSeq/MiSeq FGx effective 09/08/23 – current

mitoMPS Analysis using the Qiagen CLC Workbench and AQME Toolkit

mitoMPS Analysis using the Qiagen CLC Workbench and AQME Toolkit effective 10/28/22 – current

Interpretation Guidelines for mtDNA MPS

Statistical Analysis for Massively Parallel Sequencing

Sanger Sequencing Procedures

Mitochondrial DNA Sequencing Analysis

Mitochondrial DNA Sequencing Analysis effective 09/14/17 – current

Editing Guidelines

Editing Guidelines effective 07/08/21 – current

Sequence Nomenclature and Alignment

Sequence Nomenclature and Alignment effective 08/06/21 – current

Interpretation Guidelines

Interpretation Guidelines effective 09/12/17 – current

Statistical Analysis

Statistical Analysis effective 12/06/16 – current

Creation of a Casefile CD

Creation of a Casefile CD effective 09/12/17 – current

References

References effective 08/06/21 – current

Appendix A - Oligonucleotide Primer Sequences

Appendix A - Oligonucleotide Primer Sequences effective 06/20/16 – current

Appendix B - Mitochondrial DNA Primer Locations

Appendix B - Mitochondrial DNA Primer Locations effective 02/27/17 – current

Appendix C - Revised Cambridge Reference Sequence

Appendix C - Revised Cambridge Reference Sequence effective 06/20/16 – current