This manual contains individual procedures and protocols pertaining to Nuclear DNA testing of biological specimens. They are organized based on each step of the DNA testing process.
Procedures on this page are effective as of June 20, 2016 and later. The dates effective are shown as MM/DD/YY - MM/DD/YY in the file name.
Note: The most current manual in each section is the first link listed.
Click or press the enter key on any topic below to access the relevant PDF file of the associated procedure.
General Guidelines for Forensic Biology and DNA Casework
Automated DNAIQ Extraction from Bloodstains and Other Casework Samples
Differential Extraction from Semen Stains or Swabs Utilizing the QIAcube and EZ1
DNA Extraction of Bone Samples
Note: The DNA Extraction of Bone Samples extraction was not in use between 10/09/18 and 06/06/19.
DNA Extraction of Hair
Examination and Direct Lysis Procedure for Cartridge Casings
Extraction of Exogenous DNA from Nails
MaxSuite Automated DNA IQ Extraction from Casework Samples
Manual Appendix for MaxSuite Software
Microcon DNA Fast Flow DNA Concentration and Purification
Organic Extraction
QIAGEN EZ1 Advanced XL DNA Extraction from Bloodstains and Other Casework Samples
Qiagen Casework GO! Y Screen of Sexual Assault Stains or Swabs
Reconstitution of Evaporated Extracts
Recovery of Spotted DNA Extracts from Whatman FTA Elute MicroCards
Zygem One-Step Sperm Lysis of Sexual Assault Stains or Swabs
Chelex Extractions
These procedures were retired on 10/09/18.
High Yield DNA Extraction
This procedure was retired on 10/09/18.
MagAttract DNA Extraction Procedures
These procedures were retired on 01/09/18.
PowerPlex Fusion Direct Amplification of DNA from Buccal Swabs
PowerPlex Fusion Direct Amplification of DNA from Bloodstain Card Punches
This procedure was retired on 05/09/19
Quantifiler Trio DNA Quantification Kit
Using the Mastercycler X50s
Amplification using the Power Plex Y23 System
Amplification using the Power Plex Fusion System
Amplification using the YFiler System
This procedure was retired as of 2/3/2022.
Identifiler Sample Preparation for Amplification
This procedure was retired on 08/06/19.
Amplification using the Minifiler System
This procedure was retired on 10/10/18.
General Guidelines for Fluorescent STR Analysis
YFiler - Capillary Electrophoresis
This procedure was retired as of 2/3/2022.
PowerPlex Fusion and Y23 - Capillary Electrophoresis
GeneMarker Manual
STR Analysis on the 3500xL Genetic Analyzers
PowerPlex Fusion Ladder, Positive External Control, and Size Standard Appendix
YFiler, Identifiler, and Minifiler References - Allelic Ladders, Controls, and Size Standards
Editing Codes
ReRun Codes
GeneMarker Quality Reasons Index
Manual Appendix for PowerPlex Y23 (3500xL)
Manual Appendix for PowerPlex Fusion (3500xL)
Appendix for PowerPlex Fusion Stutter (3500xL)
Manual Appendix for PowerPlex Fusion (3130xL)
Identifiler Electrophoresis and Analysis on the ABI 3130xl Genetic Analyzer
This procedure was retired on 07/19/19.
Minifiler- Capillary Electrophoresis
This procedure was retired on 10/10/18.
Genemapper ID Analysis
Genemapper ID Analysis Method Editor Settings
Genemapper ID Default Table and Plot Settings
Genemapper ID Quality Flags
Genemapper ID Troubleshooting Guide
Interpretation of PowerPlex Y23 data run on 3500xL
Interpretation of PowerPlex Fusion data run on 3500xL
STR Results Interpretation - PowerPlex Fusion & STRmix
Additional Interpretations of Y-STR Results and Complex Y-STR Results
Sample Comparisons - Identifiler and YFiler
STR Results Interpretation - Identifiler and YFiler
Reinterpretation of Legacy Data
STRmix v 2.7 Probabilistic Genotyping Software Operating Instructions
STRmix v 2.4 Probabilistic Genotyping Software Operating Instructions
STRmix Glossary
Usage of the "Y-Mixture tool" / "Y-Mix Database Filter"
Population Frequencies for STR's
Forensic Statistical Tool
DNA View for Paternity and Kinship and Analysis
Kinship and Paternity Analysis
ANDE Rapid DNA
FlexPlex Ladder, PE, and Size Standard
Appendix
References